Notice bibliographique
- Notice
Type(s) de contenu et mode(s) de consultation : Texte noté : électronique
Auteur(s) : Casem, Merri Lynn
Titre(s) : Case studies in cell biology [Texte électronique] / Merri Lynn Casem
Publication : Amsterdam : Academic Press is an imprint of Elsevier, [2016]
Description matérielle : 1 online resource
Collection : Problem sets in biological and biomedical sciences
Note(s) : Case Studies in Cell Biology presents real world scenarios to help readers use science
process and reasoning skills. The case studies require application and analyzation
of concepts beyond rote memory of biological concepts. The book is based on the student
learning outcomes from the American Society for Cell Biology, offering practical application
for both the classroom and research laboratory.
Sujet(s) : Cytologie
Genre ou forme : Études de cas
Identifiants, prix et caractéristiques : ISBN 9780128013946
Identifiant de la notice : ark:/12148/cb451093381
Notice n° :
FRBNF45109338
(notice reprise d'un réservoir extérieur)
Table des matières : Cover; Title Page; Copyright Page; Dedication; Contents; Biography; Preface; Chapter
1 -- Introduction to Scientific Method from a Cellular Perspective; Subchapter 1.1
-- Scientific Method in Action [1]; Introduction; Background; Methods; Cell cultures;
Microinjection; Electron microscopy; Calculation of nuclear uptake and size distribution;
Results; Reference; Chapter 2 -- Cellular Biodiversity; Subchapter 2.1 -- Cellular
Biodiversity on the High Seas [1]; Introduction; Background; Methods; Cell culture
and population density; Characterization of photosynthetic pigments; Results
Subchapter 2.2 -- The Mystery of the Missing Mitochondria [2]Introduction; Background;
Methods; Subcellular fractionation of giardia; Transmission electron immunomicroscopy;
Results; References; Chapter 3 -- Proteins; Subchapter 3.1 -- Bite of the Brown Recluse
Spider: An Introduction to Protein Gel Electrophoresis [1]; Introduction; Background;
Methods; Red blood cell ghosts; Venom digestion assays; Protein gel electrophoresis;
Results; Subchapter 3.2 -- Where to Start? A Case of Too Many AUGs [3]; Introduction;
Background; Methods; Yeast culture conditions
CDC9-GFP fusions, CDC9 mutagenesis, and HA taggingSubcellular fractionation and protease
K digestion; Immunoblotting; Results; Subchapter 3.3 -- Cotranslational Translocation:
Gatekeepers of the RER [4]; Introduction; Background; Methods; In vitro translation;
Crosslinking assay; Flotation assay; Protease resistance assay; Results; Subchapter
3.4 -- From Sequence to Function [5]; Introduction; Background; Methods; Isolation
and structural analysis of bovine AQP1; Data processing; Results; Subchapter 3.5 --
Visualizing Protein Conformation [6]; Introduction; Background; Methods
Protein expression and purificationElectron microscopy; SNARE complex labeling; Results;
Subchapter 3.6 -- Second Chance Chaperones: How Misfolded Proteins get Refolded [7];
Introduction; Background; Methods; Purification of Hsp104; Purification of Hsp40 and
Hsp70; Preparation of yeast lysates; Turbidity assay; Refolding assays; Gel filtration;
Results; References; Chapter 4 -- The Nucleus; Subchapter 4.1 -- Nuclear Pore Complex
Assembly is a House of Cards [1]; Introduction; Background; Methods; Nuclear reconstitution
assay; Immunodepletion; Nuclear import; Immunoblotting; DNA replication assay
Scanning electron microscopyResults; Subchapter 4.2 -- Importin b: The Important Importin
[2]; Introduction; Background; Methods; Preparation of a high-speed supernatant from
Xenopus eggs; Immunodepletion of importin from cytosol; Nuclear protein import assay;
Nuclear localization signal binding assay; Protein gel electrophoresis and immunoblotting;
Results; Subchapter 4.3 -- A Tale of tRNA Transport [3]; Introduction; Background;
Methods; In vitro translation and 35S-labeling; Injection into Xenopus frog eggs;
In vitro binding assays; Gel electrophoresis and immunoblotting; Results
Subchapter 4.4 -- When It Comes to the Nucleus -- Size Matters [4]
