Notice bibliographique
- Notice
000 nam 22 3 450
001 FRBNF447204570000002
010 .. $a 9789048197545
035 .. $a OCoLC800193764
100 .. $a 20170801d2011 m y0frey50 ba
101 0. $a eng
102 .. $a NL
105 .. $a ||||z 00|||
106 .. $a s $a r
135 .. $a dru||||||||||
181 .0 $6 01 $a i $b xxxe
181 .. $6 02 $c txt $2 rdacontent
182 .0 $6 01 $a b
182 .. $6 02 $c c $2 rdamedia
200 1. $a Microbial Plant Pathogens-Detection and Disease Diagnosis $b Texte électronique $e Viral and Viroid Pathogens, Vol.3 $f by P. Narayanasamy
210 .. $a Dordrecht $c Springer Netherlands $c Springer e-books $d 2011
215 .. $a 1 online resource
330 .. $a Viruses, with the primitive structural features are capable of causing diseases in
all life forms on the earth. With limited morphological characteristics of the virus
particles and lack of physiological functions, the need for the development of techniques
based on the characteristics of the viral proteins and genomic nucleic acids was realized
in order to detect, identify, differentiate and quantify viruses in the infected plants/planting
materials with or without symptoms of infection. Immunoassays based on the antigenic
properties of viral capsid proteins have been successfully applied for the detection
of viruses in the symptomatic and asymptomatic crop and weed host plant species as
well as in the vectors that are involved in the transmission of the viruses infecting
crop plants. Development of monoclonal antibody technology has enhanced the sensitivity
and specificity of immunoassays especially applied for the differentiation of strains
of viruses with different virulence levels. Nucleic acid-based techniques have been
demonstrated to have higher levels of sensitivity, specificity, reliability, reproducibility
and rapidity. The viroids with only an RNA molecule have been detected by techniques
based on the biological characteristics in certain cases. Application of immunoassays
for viroid detection is not feasible because of the absence of a protein component
that is present in the viruses. Nucleic acid-based techniques are the most reliable
and sensitive tests for detection, identification and differentiation of viroids present
in plants and planting materials. With the aim of providing a comprehensive knowledge
of detection of viruses and viroids and diagnosis of the diseases caused by them,
information distilled from extensive literature search is presented to fulfil the
requirements of the researchers, teachers and graduate students of various disciplines
of biological sciences. Inclusion of numerous protocols in appropriate chapters as
appendix is a unique feature of this volume to enable the scientists to select the
suitable methods for the various investigations
676 .. $a 571.92 $v 23
801 .3 $a US $b OCoLC $c 20170801 $h 800193764 $2 marc21
801 .0 $b ZWZ $g ncafnor
930 .. $5 FR-759999999:44720457001001 $a ACQNUM-102223 $b 759999999 $c Document numérisé $d N
