Notice bibliographique
- Notice
Type(s) de contenu et mode(s) de consultation : Texte noté : électronique
Titre(s) : Guide to protein purification [Texte électronique] / edited by Richard R. Burgess, Murray P. Deutscher
Édition : 2nd ed.
Publication : Amsterdam ; Boston : Elsevier/Academic Press, 2009
Description matérielle : 1 ressource dématérialisée
Collection : Methods in enzymology ; v. 463
Note(s) : Includes bibliographical references and indexes
"The second edition of this classic Guide to Protein Purification provides a complete
update to existing methods in the field, reflecting the enormous advances made in
the last two decades. In particular, proteomics, mass spectrometry, and DNA technology
have revolutionized the field since the first edition's publication but through all
of the advancements, the purification of proteins is still an indispensable first
step in understanding their function. This volume examines the most reliable, robust
methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology,
biotechnology and sets a standard for best practices in the field. It relates how
these traditional and new cutting-edge methods connect to the explosive advancements
in the field. This "Guide to" gives imminently practical advice in order to avoid
costly mistakes in choosing a method and brings in perspective from the premier researchers
while presents a comprehensive overview of the field today. Gathers top global authors
from industry, medicine, and research fields across a wide variety of disciplines
including biochemistry, genetics, oncology, pharmacology, dermatology and immunology.
Assembles chapters on both common and less-common relevant techniques. Provides robust
methods as well as an analysis of the advancements in the field that, for an individual
investigator, can be a demanding and time-consuming process."
Autre(s) auteur(s) : Burgess, Richard R.. Fonction indéterminée
Deutscher, Murray P.. Fonction indéterminée
Sujet(s) : Protéines -- Purification
Indice(s) Dewey :
547.7 (23e éd.) = Macromolécules et composés connexes (chimie organique)
Identifiants, prix et caractéristiques : ISBN 9780123745361
Identifiant de la notice : ark:/12148/cb44634046z
Notice n° :
FRBNF44634046
(notice reprise d'un réservoir extérieur)
Table des matières : Why purify enzymes? /A. Kornberg ; Strategies and considerations for protein purifications
/S. Linn ; Use of bioinformatics in planning a protein purification /R.R. Burgess
; Preparing a purification summary table /R.R. Burgess ; Setting up a laboratory /M.P.
Deutscher ; Buffers: principles and practice /V.S. Stoll and J.S. Blanchard ; Measurement
of enzyme activity /T.K. Harris and M.M. Keshwani ; Quantitation of protein /J.E.
Noble and M.J. Bailey ; Concentration of proteins and removal of solutes /D.R. Evans,
J.K. Romero and M. Westoby ; Maintaining protein stability /M.P. Deutscher ; Selecting
an appropriate method for expressing a recombinant protein /W.H. Brondyk ; Bacterial
systems for production of heterologous proteins /S. Zerbs, A.M. Frank and F.R. Collart
; Expression in the yeast Pichia pastoris /J.M. Cregg [and others] ; Baculovirus-insect
cell expression systems /D.L. Jarvis ; Recombinant protein production by transient
gene transfer into Mammalian cells /S. Geisse and C. Fux ; Tagg
