Notice bibliographique
- Notice
Type(s) de contenu et mode(s) de consultation : Texte noté : électronique
Auteur(s) : Rosenberg, Ian M. (1949-....)
Titre(s) : Protein analysis and purification [Texte électronique] : benchtop techniques / Ian M. Rosenberg
Édition : 2nd ed.
Publication : Boston : Birkhäuser, cop. 2005
Description matérielle : 1 online resource (xxvi, 520 pages)
Note(s) : Includes bibliographical references and index
New technologies and the manner in which scientific research is conducted have been
fully incorporated into this thoroughly revised second edition of PROTEIN ANALYSIS
AND PURIFICATION. Among the many topics introduced to this new edition are the use
of robotics in the lab, the impact of the codification of the human genome, functional
proteomics, mass spectrometry, and the expanding use of bioinformatics as a tool to
improve efficiency and productivity. The author has also included an annotated appendix
of the educational materials and technical databases available online to enhance the
researcher's benchside access to the latest resources. With solid explanations of
the science asking the "why" and literally dozens of step-by-step protocols providing
the "how," this new edition is a complete resource for the benchside
Sujet(s) : Protéines -- Analyse
Protéines -- Purification
Sciences de la vie
Genre ou forme : Manuels de laboratoire
Indice(s) Dewey :
572.6 (23e éd.) = Protéines
Identifiants, prix et caractéristiques : ISBN 9780817644123
Identifiant de la notice : ark:/12148/cb446516360
Notice n° :
FRBNF44651636
(notice reprise d'un réservoir extérieur)
Table des matières : 1 An Overview of This Manual ; Kits, Cores, and Computers ; How to Use This Book
; Basic Laboratory Equipment ; Laboratory Automation ; Beyond Protein Analysis
and Purification ; 2 Protein Structure ; A. The Amino Acids ; B. The Four Levels
of Protein Structure ; C. Chemical Characteristics of Proteins ; 3 Tracking the
Target Protein ; A. Labeling Cells and Proteins ; B. LysisPreparation of the Cell
Free Extract ; C. Principles of Immunoprecipitation ; D. Additional Methods to Identify
Associated Proteins ; 4 Electrophoretic Techniques ; to Polyacrylamide Gel Electrophoresis
(PAGE) ; A. Preparation of SDS-Polyacrylamide Gels ; B. 2-Dimensional (2-D) Gel
Systems ; D. Recovery of Proteins from the Gel ; E. Identification of Enzyme Activity
in Polyacrylamide Gels ; 5 Getting Started with Protein Purification ; A. Making
a Cell Free Extract ; B. Protein Quantitation ; C. Manipulating Proteins in Solution
; D. Precipitation Techniques ; 6 Membrane Proteins ; A.
